Web1 sep. 2024 · The measure of transfection efficiency, the percentage of transfected cells in a cell population, is a subjective measure prone to many variable factors, such as cell … Web24 feb. 2024 · The cells were then centrifuged for 90 min at 1000 x g, 32 °C. Afterwards, the cells were incubated at 37 °C, 5% CO 2. The medium was exchanged daily for the …
Frontiers Construction of an Integrated mCherry Red …
Web11 apr. 2024 · First, 10 6 –10 7 mCherry-positive cells were harvested and cultured (mCherry +, Fig. 1e, left panel, named S I cells), which demonstrated that the lentiviral-integrated S variants were ... Web14 apr. 2024 · At days 3, 6 and 9 the ratio between mCherry positive and negative cells were measured by flow cytometry using LSR Fortessa (BD Biosciences) instruments, with data normalised to the NT: ... aranesa
Frontiers A Gene Transfer-Positive Cell Sorting System Utilizing ...
Web22 mrt. 2016 · Besides, cultivation in the presence of selection pressure showed that close to 100% of 6475-mCherry and R2LC-mCherry cells were positive, but that only 17% of the 6475-CBRluc-mCherry cells produced detectable amounts of fluorescence. However, the percentage increased (p<0.01), to 56%, after 10 days of subculture . WebmCherry (monomeric red fluorescent protein); labeled beads are present at 3 levels of mCherry-like intensity RFP (Red Fluorescent Protein); labeled beads are present at 3 … Web21 nov. 2024 · Three days after transfection, EGFP positive cells were sorted and cultured for an additional 4 days. Analysis of the gene integration at the DAZL locus, based on the number of mCherry positive cells, represented a 6.25 and 12.7% knock-in efficiency for HDR and HMEJ constructs, respectively (Fig. 3c). aranes